Toggle menu
Email: info@cellmosaic.com / Tel: 781-463-0002
  • LoginorSign Up
  • 0
CellMosaic
×
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
    • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
    • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up

Shop by Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
    • OxLink™ and sxLink™ Protein crosslinkers
  • Shop by Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Principle of Maleimide Kit CM90002
  • Principle of Maleimide Kit CM90002

    Maleimide Assay Kit

    MSRP:
    Now: $127.00
    (You save )
    SKU:
    CM90002
    Availability:
    In stock, usually ships within 24 hrs
    Shipping:
    Calculated at Checkout

    Adding to cart… category.add_cart_announcement
    Add to Wish List
    • Create New Wish List
    • Email
    • Print
    • Linkedin
    • Overview

    Product Description

     

    Download Documents
    Product Sheet

    Product Description

    CellMosaic’s maleimide assay kit is designed to assay the maleimide functional group content of a modified biopolymer, such as an antibody, protein, peptide, or oligo. CellMosaic routinely uses this kit for its internal bioconjugation-related research.

    The assay is based on Ellman’s assay of cysteine using 5’5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Ellman, G.L. 1959, Tissue sulfhydryl groups. Arch Biochem Biophys. 82, 70–77). First, a known amount of excess cysteine is reacted with the maleimide groups of the biopolymer, and then the unreacted cysteine is reacted with DTNB to generate 2-nitro-5-thiobenzoic acid (TNB). TNB is orange in color and has an extinction coefficient of 14,150 M-1cm-1 at 412 nm (Riddles, P.W. Blakeley, R. L., and Zerner, B. 1983, Reassessment of Ellman’s reagent. Methods Enzymol. 91, 49-60). The difference between the initial amount of cysteine and the amount of unreacted cysteine corresponds to the maleimide functional groups of the biopolymer.

    Four micro-centrifuge tubes per package. Each package is sufficient for 10 assays (100 μL per assay volume).


    Application of the product:

    • Assay the maleimide functional groups

    Key Features of the product:

    • Less than 1 h of preparation and assay time. Fast and easy to use.

     

    Product Videos

    Custom Field

    Product Reviews

    Recommended

    • SepSphere™ Peptide Immobilization Kit via Maleimide Schematic diagram of the workflow for peptide immobilization starting with 5 mL of maleimide agarose beads SepSphere™ Peptide Immobilization Kit via Maleimide Schematic diagram of the workflow for peptide immobilization starting with 5 mL of maleimide agarose beads
      Quick view Details

      SepSphere™

      |

      sku: CM71301

      SepSphere™ Peptide Immobilization Kit via Maleimide

      MSRP:
      Now: $652.00
      Choose Options
    • Maleimide Activated Ova HL Maleimide Activated Ova HL
      Quick view Details

      sku: CM52114

      Maleimide Activated Ova HL

      MSRP:
      Now: $130.00
      Choose Options
    • Thiol assay kit CM90004 Ellman's assay Thiol assay kit CM90004 Ellman's assay
      Quick view Details

      sku: CM90004

      Thiol Assay Kit

      MSRP:
      Now: $127.00
      Add to Cart
    • Ellman's assay after purification CM90005 Ellman's assay after purification CM90005
      Quick view Details

      sku: CM90005

      Thiol Assay Kit with Purification

      MSRP:
      Now: $224.00
      Add to Cart
    • Maleimide Activated KLH HL Maleimide Activated KLH HL
      Quick view Details

      sku: CM52109

      Maleimide Activated KLH HL

      MSRP:
      Now: $140.00
      Choose Options
    ×
    ×
    Join Our Mailing List for special offers!
    Contact Us
    10A Roessler Road
    Woburn, MA 01801
    781-463-0002
    info@cellmosaic.com
    Accounts & Orders
    • Wishlist
    • Login or Sign Up
    Quick Links
    • About Us
    • Contact Us
    • Products and Services
    • How to Order
    • Technologies
    • Resources
    • Careers
    Connect with Us:
    • © CellMosaic
    • |
    • Sitemap