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Oligo—Antibody Conjugate

  • Image 1
  • Scheme: thiol modified IgG reaction with maleimide activated oligo
  • Example 1: Antibody and oligo conjugates linked through a thiol ether bond.
Price:
Please contact us for a quote.
SKU:
BC0007


Product Description

  • Chemistry: Antibody and oligo conjugation through a long PEG linker and thiol ether bond formation.
  • Specification: A pool of heterogeneous products.
  • Procedure: After a buffer exchange (if necessary), labeling of amine modified-oligo with NHS ester-maleimide followed by gel filtration to remove excess crosslinking reagents. Then thiolation of antibody followed by conjugation of antibody to maleimide -activated oligo and purification. Finally, concentration determination and gel electrophoresis analysis of the oligo-antibody conjugates.
  • Price: please contact us for a quote.
  • Starting materials: Please check biopolymer for ordering information.
  • Note: In this case sulfhydryl functional groups will be introduced to an antibody through Lys residues using a reliable and reproducible procedure. However, if some of the modified or conjugated Lys residues are located on the antigen binding sites, this method may produce partially active or inactive antibody-enzyme conjugates that may not bind to the antigen. In such a case an alternative conjugation method may be used.

Examples (see images):

  1. Synthesis and purification of oligo and antibody conjugates at CellMosaic (shown below): >98% conjugates by size exclusion HPLC, characteristic UV spectrum of Ab (280 nm) and oligo (260 nm).

     

    bc0007-example.jpg

 

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