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HRP Antibody Conjugation Kit

  • HRP antibody conjugation scheme
  • HRP antibody conjugate
Price:
$360.00
SKU:
CM51406x1
Availability:
Custom kit made to order, usually ships within 1 week
Shipping:
Calculated at checkout
Quantity:


 

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SDS USER MANUAL

The kit is designed to work with antibody IgG. The customer supplies its own unmodified IgG. Using the kit components, the customer converts their antibody to a thiol-antibody, followed by reaction of the thiol-antibody with activated HRP to generate the HRP-antibody conjugates. One step purification typically provides the resulting HRP-antibody at greater than 95% purity.

This kit provides materials to label one (CM51406x1) to three antibody samples (CM51406x3). Total amount of activated HRP included in a reaction: 2 mg. 


Requirement for Antibody:

  • IgG type (for other antibody type, please contact us for a custom bioconjugation)
  • Amount: 1.2 mg per reaction (Lower amount of IgG is fine, but need customization. Please contact us for the customization)
  • Preferably >90% pure by gel electrophoresis or SEC HPLC analysis

Key Features of this HRP-Antibody Conjugation Kit:

  • High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
  • Target 1-3 HRP per antibody for a typical batch
  • A single purification step affords over 95% pure of HRP-antibody conjugates 
  • Fast preparation: less than 3 h hands-on time
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation, you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.


 Labeling Chemistry

 cm51406-hrp-antibody-scheme.png

 

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