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SDS | USER MANUAL |
This HRP-Oligo Conjugation kit works with a protected thiol-modified oligos (disulfide form). The customer supplies the thiol-modified oligos containing 3’ C6 disulfide linker or 5’ C6 disulfide linker, which are readily available from many commercial oligo suppliers. Using the kit components, the customer reduces the disulfide bond of the oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity.
This kit provides materials to label one (CM53402x1) to three oligo samples (CM53402x3). Scale of the reaction: 5 nmole oligo.
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Requirement for thiol-oligo:
Key Features of this HRP-Oligo Conjugation Kit:
Labeling Chemistry
The oligo is modified with 3’ C6 disulfide linker or 5’ C6 disulfide linker