Product Description
This kit (Cat#: CM53402) is upgraded to a new kit (Cat#: CM53408)
CM53408 has slightly different components and workflow in order to offer more flexibilities in selection of the oligo materials and improve the overall labeling efficiency.
Please click here to check out the new and upgraded replacement kit.
This HRP-Oligo Conjugation kit works with protected thiol-modified oligos (disulfide form). The customer supplies the thiol-modified oligos containing 3’ C6 disulfide linker or 5’ C6 disulfide linker, which are readily available from many commercial oligo suppliers. Using the kit components, the customer reduces the disulfide bond of the oligo, followed by reaction of the thiol-oligo with activated horseradish peroxidase (HRP) to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity.
This kit provides materials to label one (CM53402x1) or three (CM53402x3) oligo samples.
Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.)
Requirements for thiol-oligo
- Amount: 5 nmol
- The oligo is modified with 3’ C6 disulfide linker or 5’ C6 disulfide linker
- Preferably, the disulfide-oligo should be HPLC purified and lyophilized
Key Features of this HRP-Oligo Conjugation Kit
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High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
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Optimal maleimide groups per HRP for single label of oligo: 1.2 for typical batch
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A single purification step affords around 85-90% of single labeled HRP-oligo conjugates
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Fast preparation in less than 1 hour of hands-on time
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Easy purification in less than 30 minutes
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No activity loss of the HRP after the conjugation and purification
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All reagents included from preparation to purification
Labeling Chemistry
The oligo is modified with 3’ C6 disulfide linker or 5’ C6 disulfide linker
