This kit (Cat#: CM53401) is upgraded to a new kit (Cat#: CM53407)
CM53407 has slightly different components and workflow in order to offer more flexibilities in selection of the oligo materials and improve the overall labeling efficiency.
CellMosaic® has designed this HRP-Oligo conjugate with linkers and conjugation chemistry that retain the activity of HRP. The same configuration and chemistry have been tested for HRP-Oligos with different oligo sequences in several diagnostic companies since 2011.
This HRP-Oligo Conjugation kit works with amine-modified oligos. The customer supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the customer converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity.
This kit provides materials to label one (CM53401x1) to three oligo samples (CM53401x3). Scale of the reaction: 5 nmol oligo.
Key Features of this HRP-Oligo Conjugation Kit:
High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Optimal maleimide groups per HRP for single label of oligo: 1.2 for typical batch
A single purification step affords 85~90% of single labeled HRP-oligo conjugates
Fast preparation: less than 2 h hands-on time
Easy purification: less than 30 minutes
Stable linkage with optimal PEG spacer to make sure there is no interference between oligo binding and HRP activation
No activity loss of the HRP after the conjugation and purification
All reagents included, from preparation to purification
Typical Kit Performance Data (LC Analysis, CellMosaic®)
Oligo information: HPLC-purified 18mer oligo with 5’ NH2-C6 modification
Figure 1: Size exclusion chromatography (SEC) HPLC analysis of the HRP and oligo reaction mixture (before purification).
Figure 2: Size exclusion chromatography (SEC) HPLC analysis of first elution (E1) from Column Q (after purification).