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Streptavidin Oligo Conjugation Kit (Single Label with Amine Oligo)

  • Chemical Structure of Streptavidin Oligo Conjugate
  • Scheme 1: Preparation of 1:1 Streptavidin Oligo Conjugate
  • Scheme 1: Schematic Diagram of the Work Flow for Preparing 1:1 Streptavidin Oligo Conjugate
Price:
$590.00
SKU:
CM52421x1
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Quantity:


 

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SDS USER MANUAL

CellMosaic has designed this Streptavidin-oligo conjugate with linkers and conjugation chemistry that retain the activity of Streptavidin. The same configuration and chemistry has been tested for Streptavidin-oligos with different oligo sequences from a few diagnostic companies since 2011.

The kit is designed to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated Streptavidin to generate the Streptavidin-oligo conjugates. A hydrophilic PEG linker was chosen to enhance aqueous buffer solubility. The Q-column purification step typically provides the resulting Streptavidin-oligo at greater than 90% purity.

This kit provides materials to label one (CM52421x1) to three oligo samples (CM52421x3). Scale of the reaction: 5 nmol oligo.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Key Features of this AP-Oligo Conjugation Kit:

  • Offers conjugation and purification steps that primarily provide single-labeled streptavidin oligo conjugates
  • High quality maleimide activated Streptavidin for the conjugation: >99% purity 
  • Fast preparation: less than 4 h hands-on time 
  • Quality of the conjugate: >95% of labeled conjugates with majority single-labeled based on size-exclusion chromatography (SEC)
  • Stable and long flexible PEG-based linkage to minimize the interaction between AP and oligo
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for SEC and Anion exchange HPLC analysis.

 


 Labeling Chemistry

cm52421-scheme-1b.png

 


Typical Kit Performance Data (LC Analysis, CellMosaic)

Oligo information: HPLC-purified 23mer oligo with 5’ NH2-C6 modification

Kit lot number: 5528.s12.070119

Figure 1: Size-exclusion HPLC profiles of Streptavidin (blue trace), oligo (red trace), and purified product (E1) (green Trace) detected at 220 nm. SEC separates the conjugates by apparent molecular weight (MW) or size in aqueous solution. The larger the MW of the conjugate, the earlier it elutes.

cm52421-figure-1.png 

 

Table 1: Summary results

Sample Volume µL % free Streptavidin % free oligo % of Streptavidin/oligo (1:1)
E1 (first elution) 400 Not detected Not detected >90%
Recovery: 95% of the conjugates 

  

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