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    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
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    • Our Team
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  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and Others
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Shop by Category

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    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
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    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
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    • Enzyme Labeling and Conjugation Kits
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    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
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    • Analytical Standards
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    • Peptides and Peptide Conjugates
    • Mass Tags and Labeling Reagents
    • Modified Biomolecules and Conjugates
    • Others
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    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
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  • Protein/Enzyme/Antibody Labeling through Surface Amine
  • General scheme for amine coupling with an NHS ester
  • Example 1: Fluorescent labeling of an antibody with a defined DOL for assay development
  • Example 2: Fluorescent labeling of a protein with a defined DOL for assay development
  • Example 3: Biotinylation of a protein with a defined DOL for assay development
  • Protein/Enzyme/Antibody Labeling through Surface Amine General scheme for amine coupling with an NHS ester Example 1: Fluorescent labeling of an antibody with a defined DOL for assay development Example 2: Fluorescent labeling of a protein with a defined DOL for assay development Example 3: Biotinylation of a protein with a defined DOL for assay development

    Protein/Enzyme/Antibody Labeling through Surface Amine

    Custom synthesis, please contact us for a quote.

    (You save )
    SKU:
    CSBL01

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    Product Description

    Proteins/enzymes/antibodies in their native form usually contain large numbers of surface amine groups (Lys or N-terminal amines). Those surface amine groups can easily react with an acylating reagent, such as NHS-ester or isothiocyanate activated small molecule, to form a stable amide bond or equivalent. The products obtained usually contain multiple labels per molecule. Depending on the nature of the small molecules, total pI of the protein/enzyme/antibody may be altered after labeling.


    CellMosaic has Personalized Conjugation Kits (PerKit™) for protein/enzyme/antibody labeling and conjugation, please click here to learn our PerKit™ product line.

    For large scale or project beyond the scope of PerKit™ configuration, please contact us for a quote.


    Examples (see images):

    1. Fluorescent labeling of a therapeutic antibody with defined DOL for assay development at CellMosaic (shown below): over 99.9% pure by size exclusion HPLC.

      bl002example.jpg

    2. Fluorescent labeling of a protein with defined DOL for assay development at CellMosaic (shown below): over 97.5% pure by size exclusion HPLC, a comprehensive bioanalytical tools were used to characterize the product.

      bl002example3-flu.jpg

    3. Biotinylation of protein at CellMosaic (shown below): only 52% pure starting protein was used and over 99.9% pure of biotinylated protein was isolated.

      bl002-protein-biotinylation.jpg

    References:

    1. Jobbagy, A.; Kiraly K. Chemical characterization of fluorescein isothiocyanate-protein conjugates. Biochim Biophys Acta 1966 Jul 27;124(1):166-75. PMID: 4165223.
    2. Bragg, P.D.; Hou, C. Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium.Arch Biochem Biophys. 1975 Mar;167(1):311-21. PMID: 124154.
    3. Lomant, A. J.; Fairbanks, G. Chemical probes of extended biological structures: synthesis and properties of the cleavable protein cross-linking reagent [35S]dithiobis(succinimidyl propionate). J Mol Biol. 1976 Jun 14;104(1):243-61.PMID: 957432.

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