Proteins/enzymes/antibodies in their native form usually contain large numbers of surface amine groups (Lys or N-terminal amines). Those surface amine groups can easily react with an acylating reagent, such as NHS-ester or isothiocyanate activated small molecule, to form a stable amide bond or equivalent. The products obtained usually contain multiple labels per molecule. Depending on the nature of the small molecules, total pI of the protein/enzyme/antibody may be altered after labeling.
CellMosaic has Personalized Conjugation Kits (PerKit™) for protein/enzyme/antibody labeling and conjugation, please click here to learn our PerKit™ product line.
For large scale or project beyond the scope of PerKit™ configuration, please contact us for a quote.
Examples (see images):
References: