Product Description
This MALDI-TOF MS analysis is suitable for intact antibody-drug conjugates (ADCs) and other protein conjugates with molecular weights up to 200 KDa. In addition to ADCs, CellMosaic® routinely analyzes a variety of conjugates, including protein–drug conjugates (PDCs), BSA or ovalbumin (OVA) small-molecule conjugates, and HRP small-molecule conjugates.
When a conjugate sample is submitted alongside its corresponding unlabeled protein or antibody (counted as two samples), the resulting data can be used to determine the conjugation loading ratio. A standard PDF report including the MS profile and calculated loading will be provided.
Pricing is charged on a per-sample basis. Standard turnaround time is 1–2 weeks.
Sample submission for Ab, ADC, protein and their conjugates:
1. For protein/antibody conjugates, provide up to 100 µL (≥100 µg) of ADC or other protein conjugate sample (solution or solid) in a screw-cap microcentrifuge tube.
2. Also provide an unlabeled protein/antibody sample for DAR calculation: up to 50 µL (≥50 µg) (2 samples total).
3. If the sample is lyophilized, provide ≥100 µg of lyophilized powder directly.
4. ADDITIONAL CHARGES MAY APPLY: Standard pricing includes one desalting step. Samples requiring special handling, such as concentration, will incur an additional sample handling fee of $310/hour.
Please provide samples at the highest possible concentration. Additionally, include the drug-linker molecular weight and the mass increase resulting from attachment of one drug-linker molecule.
5. Please download and complete the Sample Submission Form with all required information. Email a copy, and include a printed copy with your samples. Ship with a cold pack via overnight or earliest delivery.
Example of MALDI-TOF Analysis
ADC Information: CM51102: ADC Control hIgG1-SN38, Lot#: S412.S13.0801B, ~3.2 DAR
Figure 1: MALDI-TOF Mass Analysis of Antibody before (Left) and after conjugation with SN38 (Right) using ADC Control hIgG1-SN38 (CM51102) as an example.
