Product Description
This MALDI-TOF MS analysis is suitable for intact antibody-drug conjugates (ADCs) and other protein conjugates with molecular weights up to 200 KDa. In addition to ADCs, CellMosaic® routinely analyzes a variety of conjugates, including protein–drug conjugates (PDCs), BSA or ovalbumin (OVA) small-molecule conjugates, and HRP small-molecule conjugates.
When a conjugate sample is submitted alongside its corresponding unlabeled protein or antibody (counted as two samples), the resulting data can be used to determine the conjugation loading ratio. A standard PDF report including the MS profile and calculated loading will be provided.
Pricing is charged on a per-sample basis. Standard turnaround time is 1–2 weeks.
Sample submission for Ab, ADC, protein and their conjugates:
1) Provide ≥100 μg of ADC or other protein conjugate samples (solid or solution) in a screw-cap microcentrifuge tube. An unlabeled antibody or protein sample is also required for DAR (drug-to-antibody ratio) calculation (total of 2 samples). Additionally, provide the molecular weight of the drug-linker.
2) Please download and complete the Sample Submission Form with all required information. Email a copy, and include a printed copy with your samples. Ship with a cold pack via overnight or earliest delivery.
Example of MALDI-TOF Analysis
ADC Information: CM51102: ADC Control hIgG1-SN38, Lot#: S412.S13.0801B, ~3.2 DAR
Figure 1: MALDI-TOF Mass Analysis of Antibody before (Left) and after conjugation with SN38 (Right) using ADC Control hIgG1-SN38 (CM51102) as an example.
