Product Description
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CellMosaic’s Thiol Assay Kit with Purification is designed to assay the free thiol groups of a biopolymer after reducing or labeling with small molecule thiol-modification reagents. CellMosaic routinely uses this kit for its internal bioconjugation-related research.
This product can be used together with our antibody or other biopolymer labeling and conjugation kits to optimize loading. Examples of these kits include:
- Antibody MMAE Conjugation Kit (With VC-PAB Linkage) (CM11409 and CM11409x3)
- Antibody Mc-MMAF Conjugation Kit (CM11422 and CM11422x3)
- Antibody MMAF Conjugation Kit (with VC-PAB Linker) (CM11425 and CM11425x3)
- F(ab')2 MMAE Conjugation Kit (With VC-PAB Linkage) (CM11416 and CM11416x3)
Please follow the protocols for using the thiol assay kit along with these conjugation kits.
About the Assay:
The assay is based on Ellman’s assay using 5’5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Ellman, G.L. 1959, Tissue sulfhydryl groups. Arch Biochem Biophys. 82, 70–77). Thiol is reacted with DTNB to generate 2-nitro-5-thiobenzoic acid (TNB). TNB is orange in color and has an extinction coefficient of 14,150 M-1cm-1 at 412 nm (Riddles, P.W. Blakeley, R. L., and Zerner, B. 1983, Reassessment of Ellman’s reagent. Methods Enzymol. 91, 49-60). The amount of thiol groups will be calculated based on the amount of TNB generated.
Application of the product:
- Purification of a small amount of biopolymer after a reducing or thiol labeling reaction
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Assay the free thiol groups
Key Features of the product:
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Less than 30 minutes of preparation and assay time. Fast and easy to use.
- Each package is sufficient for 10 purifications and 5 assays (100 μL per assay volume).
Frequently Asked Questions:
The list below includes FAQs that are specific to this product.
Question: What is the difference between CM90004 and CM90005?
Answer: CM90004 (thiol assay kit) is for checking purified biopolymer containing a thiol group. CM90005 (thiol assay kit with purification) is for assaying thiol groups after biopolymer modification. This kit includes a purification spin column to remove unreacted reducing reagents or thiolation reagents before the assay.
If you can’t find the answer you’re looking for or need information on general topics, please visit the main Frequently Asked Questions (FAQs) section.
