Chemical Information

 Protein A immobilized on agarose 4B beads through isourea linkage.

Specification

• Ligand: recombinant protein A (manufactured in E. coli with animal-free materials)
• Physical appearance: suspension in 0.02% NaN3 solution.
• Immobilization method: immobilized through the reaction of the primary amines of protein A and hydroxyl groups of agarose.
• Storage temp.: 2- 8 °C
• Ligand concentration: ~ 5 mg protein A per mL of settled beads
• Available binding capacity: ~ 42 mg human IgG per ml of settled beads 
• Matrix: agarose 4B (source: GE Life Sicence).
• pH stability: Long term: 3 to 9, short term: 2 to 10
• Particle size: 45-165 mm

Notes

CellMosaic's SepSphere™ protein A agarose has been used routinely in house for purification of antibodies and antibody conjugates, demonstrating consistent performance and quality.

Figure 1: Binding Specificity of Protein A Agarose.

10 µl Protein A Agarose beads were incubated at RT for 2 hours with 40 µl PBS buffer containing 1 µg/µl of human IgG and 0.5 µg/µl of thyroglobumin, bovine serum albumin, ovalbumin, carbonic anhydrase, and RNase A each. The input and supernants (5 ul) were run on a 4-12% Bis-Tris SDS-PAGE (Invitrogen) with 1X MES running buffer and stained with Simply Blue (Sigma).

• I: Input
• A: supernatant of Protein A Agarose beads
• B: supernatant of Agarose beads