Alprenolol is an antagonist for ß1 and ß2 adrenergic receptors. Alprenolol agarose affinity chromatography was first introduced by Lefkowitz R. J. in 1979 for the purification of erythrocyte ß-adrenergic receptor based on ligand-receptor interactions. It is now routinely used for the purification of functionally active ß1 and ß2-adrenergic receptor.
Alprenolol immobilized on agarose 4B beads through thiol ether linkage.
- Ligand: alprenolol
- Physical appearance: suspension in 0.02% NaN3 solution.
- Immobilization method: immobilized through the reaction of the double bond of alprenolol and S-alkyl-thiosulfate agarose.
- Storage temp.: 2- 8 °C
- Ligand concentration: 1 ~ 3 uMole per mL of settled beads by thiol assay
- Matrix: agarose 4B (source: GE Life Science).
- Spacer: 12 atoms
- Particle size: 45-165 um
- Shorr, R., G.; Lefkowitz, R. J.; Caron, M. G. Purification of the ß-adrenergic receptor. J. Bio. Chem. 1981, 256(11), 5820-5826; 2. Caron, M. G.; Srinivasan, Y.; Pitha, J.; Kociolek, K.; Lefkowitz, R. J. Affinity chromatoraphy of the ß-adrenergic receptor J. Bio. Chem. 1979, 254(8), 2923-2927.