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    • SepSphere™ Alprenolol Agarose
  • Chemical Structure of alprenolol agarose provided in CM29101.
  • CM29101 SepSphereâ„¢ Alprenolol agarose tube packaging.
  • Chemical Structure of alprenolol agarose provided in CM29101. CM29101 SepSphereâ„¢ Alprenolol agarose tube packaging.

    SepSphere™ Alprenolol Agarose

    SepSphere™

    MSRP:
    Now: $279.00
    (You save )
    SKU:
    CM29101-1ML
    Availability:
    In stock, usually ships within 24 hrs
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    Product Description

    Download Documents
    SDS Product Sheet
    COA-050721

    Alprenolol is an antagonist for ß1 and ß2 adrenergic receptors. Alprenolol agarose affinity chromatography was first introduced by Lefkowitz R. J. in 1979 (References 1 and 2) for the purification of erythrocyte ß-adrenergic receptor based on ligand-receptor interactions. It is now routinely used for the purification of functionally active ß1 and ß2-adrenergic receptor. CellMosaic’s SepSphere™ Alprenolol Agarose  is composed of Agarose 4B Beads linked to alprenolol via a thiol ether linkage.  


    Chemical Information

     Alprenolol immobilized on agarose 4B beads through thiol ether linkage.


    Specification

    • Ligand: alprenolol
    • Physical appearance: suspension in 0.02% NaN3 solution.
    • Immobilization method: immobilized through the reaction of the double bond of alprenolol and S-alkyl-thiosulfate agarose.
    • Storage temp.: 2- 8 °C
    • Ligand concentration: 1 ~ 3 uMole per mL of settled beads by thiol assay
    • Matrix: agarose 4B (source: GE Life Science).
    • Spacer: 12 atoms
    • Particle size: 45-165 um

     References

    1. Shorr, R., G.; Lefkowitz, R. J.; Caron, M. G. Purification of the ß-adrenergic receptor. J. Bio. Chem. 1981,  256(11),  5820-5826;
    2. Caron, M. G.; Srinivasan, Y.; Pitha, J.; Kociolek, K.; Lefkowitz, R. J. Affinity chromatoraphy of the ß-adrenergic receptor J. Bio. Chem. 1979,  254(8),  2923-2927.

    Citations from Customer Who Used Our Product:

    1. Cheong, Hoi I.; Asosingh, Kewal; Stephens, Olivia R.; Queisser, Kimberly A.; Xu, Weiling; Willard, Belinda; Hu, Bo; Kam Tai Dermawan, Josephine; Stark, George R.; Naga Prasad, Sathyamangla V.; Erzurum, Serpil C.; Hypoxia sensing through β-adrenergic receptors. JCI Insight. 2016;1(21):e90240. https://doi.org/10.1172/jci.insight.90240.

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