Product Description
| Download Documents | |
| SDS | USER MANUAL |
Ovalbumin (Ova) is a small protein sometimes used as a carrier protein for antibody production. Chicken egg albumin is the major protein constituent of egg whites and has a MW of 44.3 KDa if the carbohydrate and phosphate portions are accounted. CellMosaic® has designed this personalized Ova conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, customers prepare the Ova-peptide conjugate by reacting their peptide (supplied by the customer) with activated Ova (supplied in the kit). A single purification step typically provides the resulting Ova-peptide at greater than 95% purity.
This kit provides materials to perform Ova labeling of one (CM52418x1) or three peptides (CM52418x3). Total amount of activated Ova included in a reaction: 2 mg.
Requirement for Cys peptide:
- Amount: 0.23–0.9 µmol (5–20 equivalents of peptide over Ova).
- HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
- The Cys peptide should be stored at -80°C .
- HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
Key Features of this Ova-Peptide Conjugation Kit:
-
High quality maleimide-activated Ova for the conjugation: >95% purity
-
High maleimide groups per Ova for peptide loading: 10-20
-
A single purification affords over 95% of the Ova-peptide conjugates
-
Fast preparation: less than 1 hour hands-on time
-
All reagents included, from preparation to purification
- Options to choose tailored services at CellMosaic® prior to and after conjugation
Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);
After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.
Frequently Asked Questions:
The list below includes FAQs that are specific to this product.
Question: My peptide sequence has two cystine residues. Will I be able to use the Ova-Peptide Conjugation Kit?
Answer: With two active cysteines you may run into the situation where your peptide may crosslink two ova together. However, as this kit is designed to produce relatively high loading peptide-ova conjugates, the dimerization and polymerization will be relatively difficult to form because the priority will be to form the Ova-Peptide conjugate and use up all of the active maleimide groups. For modifications to the protocol to help with specific features of your peptide we offer a PerKit sheet, where we write a modified protocol for you.
If you can’t find the answer you’re looking for or need information on general topics, please visit the main Frequently Asked Questions (FAQs) section.
