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    • Peptide and Protein
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    • KLH-Peptide Conjugation Kit
  • Chemical Structure of Peptide KLH conjugates generated using CM52411 PerKit KLH-Peptide Conjugation Kit
  • KLH Peptide Conjugation chemistry. Reaction scheme used in CM52411 PerKit KLH-Peptide Conjugation Kit
  • Schematic diagram of the workflow used to generate KLH Peptide conjugates using CM52411 PerKit KLH-Peptide Conjugation Kit
  • Chemical Structure of Peptide KLH conjugates generated using CM52411 PerKit KLH-Peptide Conjugation Kit KLH Peptide Conjugation chemistry. Reaction scheme used in CM52411 PerKit KLH-Peptide Conjugation Kit Schematic diagram of the workflow used to generate KLH Peptide conjugates using CM52411 PerKit KLH-Peptide Conjugation Kit

    KLH-Peptide Conjugation Kit

    PerKit®

    MSRP:
    Now: $374.00
    (You save )
    SKU:
    CM52411
    Availability:
    In stock, usually ships within 1-3 days
    Shipping:
    Calculated at Checkout
    Reaction Scale:
    0.10–0.51 µmol of Peptide (2 mg of KLH)

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    Product Description

    Download Documents
    SDS USER MANUAL
    COA_Activated KLH Lots S337.S14.052521

    Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, our customers prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single step purification process then typically provides the resulting KLH-peptide conjugate at greater than 95% purity.

    This kit provides materials to perform labeling of KLH with one (CM52411x1) or three (CM52411x3) peptides. Total amount of activated KLH included in a reaction: 2 mg.


    Requirement for Cys peptide:

    • Amount: 0.10–0.51 µmol (20–100 equivalents of peptide over KLH)
    • HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
    • The Cys peptide should be stored at -80°C.
    • HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

    Key Features of this KLH-Peptide Conjugation Kit:

    • High quality maleimide-activated KLH for the conjugation: >95% purity 
    • High maleimide groups per KLH for peptide loading: 40-100
    • A single purification step affords over 95% of the KLH-peptide conjugates 
    • Fast preparation: less than 1 hour hands-on time
    • All reagents included from preparation to purification
    • Options to choose tailored services at CellMosaic® prior to and after conjugation

    Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);

    After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.

    Product Videos

    Custom Field

    Reaction Scale 0.10–0.51 µmol of Peptide (2 mg of KLH)

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