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    • Assay, Buffers and Others
    • Hydrophobic Interaction Chromatography (HIC) Buffer Set
  • Two 500 mL bottles containing buffer A (left) and buffer B (right) of the HIC Buffer Set.
  • Two 500 mL bottles containing buffer A (left) and buffer B (right) of the HIC Buffer Set.

    Hydrophobic Interaction Chromatography (HIC) Buffer Set

    MSRP:
    Now: $296.00
    (You save )
    SKU:
    CM02025-250ML
    Availability:
    In stock, usually ships within 24 hrs
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    Product Description

    Download Documents
    SDS Product Sheet
    COA_CM02025.1_Buffer A_0321C
    COA_CM02025.1_Buffer B_0321C

    HIC HPLC analysis is a useful tool to separate bioconjugates labeled with very hydrophobic small molecules based on their hydrophobicity. The hydrophobic interaction of the bioconjugates and the column media is affected by the presence of salts or organic solvent in the running buffer.

    CellMosaic® has designed this high quality HIC buffer set for your convenient analysis of bioconjugates, such as antibody-drug conjugate (ADC) and protein or peptide-drug conjugate following the preparation of the bioconjugates using CellMosaic’s Personalized Conjugation Kit™ (PerKit™).

    This HIC buffer set comes with two buffers and instruction for usage. Buffer A has high salt and Buffer B contains no salt. A high salt concentration aids the interaction of the bioconjugates with the column media. Usually, a decreasing salt gradient is used to elute samples from the column. As such, the lowest degree of labeled bioconjugate (low hydrophobicity) is eluted first and the most labeled bioconjugates elute last.


    Quality and Convenience

    • Experimentally selected ultra-pure components for minimum UV background absorbance.
    • Sterile filtrated & bottled to prevent any analyte degradation during the run
    • Can be used with any standard HIC column for biopolymer analysis
    • Each buffer set is sufficient for 30, 60, or 120 analysis

    Application of the product:

    • Analyze antibody-drug conjugates
    • Analyze protein and peptide-drug conjugates
    • Analyze other biopolymers labeled with very hydrophobic small molecules

    Reference: Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products. Szabolcs Feketea; Jean-Luc Veuthey; Alain Beck; Davy Guillarme. Journal of Pharmaceutical and Biomedical Analysis 130 (2016) 3–18. 


    Key Components:

    Two buffer bottles per package. Number of Injections per buffer set: CM02025-250 mL (30 injections); CM02025-500 mL (60 injections); CM-02025-1L (120 injections).. 

    Name

    Part #

    Quantity

    Description

    Buffer A (Red label)

    CM02025.1

    250 mL, 500 mL, or 1L

    0.1M Sodium phosphate buffer, 1.8 M Ammonium sulfate, pH 7.0

    Buffer B (Blue label)

    CM02025.2

    200 mL, 400 mL, or 800 mL

    0.1M Sodium phosphate buffer, pH 7.0

     

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