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Fab MMAE Conjugation Kit (With VC-PAB Linkage)

  • Fab MMAE conjugate CM11415
  • MC-VC-PAB MMAE chemical structure
Price:
$990.00
SKU:
CM11415
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Quantity:


 

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SDS USER MANUAL CM11415
Kit Performance Data Lot# 072519

CellMosaic has designed this kit for labeling any Fab with monomethyl auristatin E (MMAE) using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker for early stage screening of your antibody. 

The user supplies their own unmodified Fab. Using the kit components, the user converts the Fab to a thiol-Fab via surface amine labeling, followed by reaction of the thiol-Fab with maleimide-activated VC-PAB-MMAE (included in the kit) to generate Fab-MMAE conjugates. The product is purified to remove any unreacted drug. 

Kit is configured to label either one or three antibodies. Reaction scales: 1 mg

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Drug Information

  • Name: Monomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
  • CAS number: 646502-53-6
  • Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
  • Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

Requirement for Fab

  1. Preferably >90% pure by gel electrophoresis
  2. Total amount: 1 mg (protein content measured by UV. If you don't have the exact amount, please contact CellMosaic for customization.)

Key Features of this PerKit™

  • Offers a simple and easy way to label Fab with MMAE with minimum exposure to the toxin
  • Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
  • Fast and easy preparation: 6 h preparation and <2 h hands-on time
  • All reagents and supplies included for preparation and purification
  • Target average 2-4 MMAE per Fab molecule
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DAR.


Conjugation Chemistry

cm11415-conjugation-chemistry.png


Protocol

protocol-cm11415.png


Related Products

Thiol Assay Kit (Cat. CM90004)The average free thiol groups per antibody is 4 after reducing. If necessary, you can purchase this kit separately from CellMosaic to measure the free thiols. Total assay time is 20 minutes.

PerKit™ Fab Preparation Kit (Cat. CM51407)

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