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Fab MMAE Conjugation Kit (With VC-PAB Linkage)

  • Fab MMAE conjugate CM11415
  • MC-VC-PAB MMAE chemical structure
In stock, usually ships within 1-3 days
Calculated at checkout


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Kit Performance Data Lot# 072519

CellMosaic has designed this kit for labeling any Fab with monomethyl auristatin E (MMAE) using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker for early stage screening of your antibody. 

The user supplies their own unmodified Fab. Using the kit components, the user converts the Fab to a thiol-Fab via surface amine labeling, followed by reaction of the thiol-Fab with maleimide-activated VC-PAB-MMAE (included in the kit) to generate Fab-MMAE conjugates. The product is purified to remove any unreacted drug. 

Kit is configured to label either one or three antibodies. Reaction scales: 1 mg

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Drug Information

  • Name: Monomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
  • CAS number: 646502-53-6
  • Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
  • Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

Requirement for Fab

  1. Preferably >90% pure by gel electrophoresis
  2. Total amount: 1 mg (protein content measured by UV. If you don't have the exact amount, please contact CellMosaic for customization.)

Key Features of this PerKit™

  • Offers a simple and easy way to label Fab with MMAE with minimum exposure to the toxin
  • Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
  • Fast and easy preparation: 6 h preparation and <2 h hands-on time
  • All reagents and supplies included for preparation and purification
  • Target average 2-4 MMAE per Fab molecule
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DAR.

Conjugation Chemistry




Related Products

Thiol Assay Kit (Cat. CM90004)The average free thiol groups per antibody is 4 after reducing. If necessary, you can purchase this kit separately from CellMosaic to measure the free thiols. Total assay time is 20 minutes.

PerKit™ Fab Preparation Kit (Cat. CM51407)

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