Toggle menu
781-463-0002
  • LoginorSign Up
  • 0
CellMosaic
×
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up

Shop by Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop by Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • AqT™ Biotin Active Ester Antibody Labeling Kit (T2A15)
  • AqT™ Biotin Active Ester Antibody Labeling Kit (T2A15)

    AqT™ Biotin Active Ester Antibody Labeling Kit (T2A15)

    PerKit™

    MSRP:
    Now: $167.00
    (You save )
    SKU:
    CM86140.1
    Availability:
    In stock
    Shipping:
    Calculated at Checkout
    Scale of the Reaction:
    10 ug-3 mg
    Antibody Labeling Sites:
    Surface amines

    Adding to cart… category.add_cart_announcement
    Add to Wish List
    • Create New Wish List
    • Email
    • Print
    • Linkedin
    • Overview

    Product Description

     

    Download Documents
    SDS USER MANUAL CM86140

    AqT™ Biotin Active Ester Antibody Labeling kit (T2A15) is a proprietary biotinylation kit developed at CellMosaic for obtaining high quality and high loading of biotinylated antibody with minimal or no aggregation. The kit uses a biotin modified with a super-hydrophilic, water-soluble, and charge neutral AqueaTether™ (AqT™) T2A15 linker to improve its water solubility and decrease its non-specific interaction with other proteins or plastic surfaces. Biotinylated antibodies with AqT™ linker can largely retain its native properties. AqT™ T2A15 linker composed of two threitols and 15 atom lengths. This atom length is equivalent to PEG4 (16 atom lengths) and, based on our experience, can largely keep the bivalent molecule function independent. The conjugates we have tried with this atom length include antibody oligo, streptavidin oligo, and HRP oligo, among others.

    For a detailed description of the AqT™ technologies, please see our technology section.

    The CM86140 kit is designed to biotinylate any antibody via surface amines of the antibody. The user supplies the antibody. Using the kit components, the user biotinylates their antibody in one simple step, followed by filtration-type purification to remove any unreacted AqT™-biotin, exchanging it with PBS or other buffer for downstream application. A quick 10-minute biotin assay (protocol and reagent) is also included in the kit to obtain the biotinylation level immediately after labeling. The biotinylation level can easily be tuned by increasing or decreasing the reaction temperature. The hands-on time is less than 30 minutes, and the whole preparation time varies from 3 h to overnight depending on the reaction time.

    The kit has multiple configurations for labeling various amounts of antibody (10 μg to 3 mg) with either one (x1) or three (x3) antibody samples. 

    Catalog Number

    Number of Reactions in Kit

    Antibody Amount per Reaction

    CM86140.1x1

    1

    10 μg

    CM86140.1x3

    3

    CM86140.2x1

    1

    50 μg

    CM86140.2x3

    3

    CM86140.3x1

    1

    100 μg

    CM86140.3x3

    3

    CM86140.4x1

    1

    1 mg

    CM86140.4x3

    3

    CM86140.5x1

    1

    3 mg

    CM86140.5x3

    3

     


    CM86140 and Its Product Compared to Other Commercial Antibody Biotinylation Kits

     

     

    AqT™ Biotin Labeling Kit

    Other Commercial Kits

    Biotinylated Product Features

     

    Chemical structure

    Fully Disclosed

    ·  Complete knowledge of your product

    Varies by vendor, mostly not disclosed.

    Properties

    Retain antibody properties

    ·  No changes in hydrophobicity

    ·  No increase of apparent MW (hydrodynamic volume)

    ·  No increase in aggregation

    ·  Minimal heterogeneity after labeling

    Change antibody properties

    ·  Increased hydrophobicity (non-specific interaction)

    ·  Increased apparent MW (hydrodynamic volume)

    ·  May increase aggregation

    ·  Highly heterogeneous product

    Stability

    Stable even with high biotin loading

    Can be easily stored frozen and in lyophilized form without any stabilizer

    Decreased stability

    High biotin loading may cause aggregation and precipitation

    Kit Features

    Reagent

    Proprietary water-soluble pre-activated biotin with 15-atom spacer

    Biotin (zero length) or biotin with hydrophobic ethylene- or polyethylene glycol-type linker

    Labeling buffer

    100% aqueous buffer

    Some percentage of organic solvent needed, such as DMSO

    Biotinylation level

    Predictable

    ·  Tunable loading, from low to very high, per your need

    Unpredictable

    Usually target low labeling to avoid aggregation (difficult to tune)

    Usability

    Easy to use

    ·  One step labeling

    ·  <30 minutes hands-on time

    Comparable quick labeling may use EDC chemistry with antibody crosslinking to each other causing large aggregates

    Purification method & components

    Included

    Product is free of any unreacted AqT™ biotin after purification.

    Varies by vendor, usually not included

    Biotin Assay

    Included

    A quick 10-minute biotin assay protocol with reagents and buffer

    Not included


    Requirements for antibody:

    • MW: ≥ 100 KDa (if MW is lower, please use an alternative kit)
    • Purity: preferably > 90% pure by gel electrophoresis
    • Amount: 10 μg to 3 mg protein content as measured by UV 

     

    Labeling Chemistry

    cm86140-biotinylation-chemistry.png


    Overview of the workflow for preparing AqT™ antibody-biotin conjugates

    cm86140-protocol.png

     


    Comparing the hydrophilicity of AqT™ biotin-T2A15 acid with biotin and biotin-PEG4 acid (C18 HPLC Profile)

    overall-aqt-biotin-full-length.png


    Comparing the hydrophilicity and heterogenicity of AqT™ biotin-T2A15 labeled antibody with unmodified biotin labeled antibody (HIC HPLC Profile)

     aqt-technology-page-figure5.png


    Comparing the apparent MW (hydrodynamic volume) of AqT™ biotin-T2A15 labeled antibody with unmodified biotin labeled antibody (SEC HPLC Profile)

    aqt-technology-page-figure6.png

    Product Videos

    Custom Field

    Scale of the Reaction 10 ug-3 mg
    Antibody Labeling Sites Surface amines

    Product Reviews

    Recommended

    • HRP antibody conjugate HRP antibody conjugate HRP antibody conjugate HRP antibody conjugate
      Quick view Details

      PerKit™

      |

      sku: CM51406x1

      HRP Antibody Conjugation Kit

      MSRP:
      Now: $620.00
      Choose Options
    • Streptavidin Antibody Conjugation Kit Work Flow for Preparing streptavidin-antibody conjugates Streptavidin Antibody Conjugation Kit Work Flow for Preparing streptavidin-antibody conjugates
      Quick view Details

      PerKit™

      |

      sku: CM52420x1

      Streptavidin Antibody Conjugation Kit

      MSRP:
      Now: $629.00
      Choose Options
    • Antibody SN38 (7-ethyl-10-hydroxycamptothecin) Conjugation Kits Antibody SN38 Conjugation Kit Boxes Antibody SN38 (7-ethyl-10-hydroxycamptothecin) Conjugation Kits Antibody SN38 Conjugation Kit Boxes
      Quick view Details

      PerKit™

      |

      sku: CM11408

      Antibody SN38 Conjugation Kit

      MSRP:
      Now: $1,124.00
      Choose Options
    • Chemical structure of light and heavy quaternary amine (LQA and HQA) Quaternary Amine MS-tag carboxylic acid labeling kit protocol Chemical structure of light and heavy quaternary amine (LQA and HQA) Quaternary Amine MS-tag carboxylic acid labeling kit protocol
      Quick view Details

      sku: CM63401

      Light and Heavy Quaternary Amine MS Labeling Kit for Carboxylic Acid

      MSRP:
      Now: $225.00
      Add to Cart
    • AqT™ Biotin G/H IgG, Fc Gamma Fragment Specific AqT™ Biotin G/H IgG, Fc Gamma Fragment Specific
      Quick view Details

      PerKit™

      |

      sku: CM86101

      AqT™ Biotin G/H IgG, Fc Gamma Fragment Specific

      MSRP:
      Now: $202.00
      Add to Cart
    ×
    ×
    Join Our Mailing List for special offers!
    Contact Us
    10A Roessler Road
    Woburn, MA 01801
    781-463-0002
    info@cellmosaic.com
    Accounts & Orders
    • Wishlist
    • Login or Sign Up
    Quick Links
    • About Us
    • Contact Us
    • Products and Services
    • How to Order
    • Technologies
    • Resources
    • Careers
    Connect with Us:
    • © CellMosaic
    • |
    • Sitemap