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    • Surface Activation

    Activation of a solid support is often an intermediate step required before a biopolymer can be immobilized. Some solid supports are commercially available in an activated form. For non-activated solid supports, CellMosaic can either convert the material into an activated intermediate or perform immobilization directly using appropriate coupling reagents.

    These activation processes are used exclusively to support our custom Biopolymer Immobilization services.

    Example 1. Activation of Hydroxyl-Containing Solid Supports Using Cyanogen Bromide

    Hydroxyl-containing solid supports, such as agarose, can be activated with cyanogen bromide (CNBr) to generate reactive cyanate ester intermediates for subsequent coupling to primary amine-containing biopolymers or small molecules.

    Because activated cyanate esters are relatively unstable, CellMosaic typically prepares the activated solid support immediately before large-scale immobilization of antibodies, antigens, and other biopolymers.

    Activation of hydroxyl-containing solid supports using cyanogen bromide

    Scheme 1. Activation of hydroxyl-containing solid supports using cyanogen bromide (CNBr).

    References

    1. Axén, R.; Porath, J.; Ernback, S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967, 214, 1302–1304.
    2. Porath, J.; Aspberg, K.; Drevin, H.; Axén, R. Preparation of cyanogen bromide-activated agarose gels. J. Chromatogr. 1973, 86, 53–65.
    3. March, S. C.; Parikh, I.; Cuatrecasas, P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal. Biochem. 1974, 60, 149–152.
    4. Kohn, J.; Wilchek, M. A colorimetric method for monitoring activation of Sepharose by cyanogen bromide. Biochem. Biophys. Res. Commun. 1978, 84, 7–14.