CellMosaic's SepSphereÔ protein A agarose is a high-quality affinity resin for antibody purification, fractionation, and immunoprecipitation. SepSphereÔ protein A agarose is prepared by covalently coupling purified recombinant protein A to 4% crosslinked agarose beads using a cyanogen bromide activation method. Protein A binds specifically to the Fc region of immunoglobulins, leaving the Fab regions free for binding antigens. As a result of this property, Protein A Agarose has been used to isolate and purify immunoglobulins and immune complexes from serum, ascites, tissue culture supernatants, and other biological fluids.
Protein A immobilized on agarose 4B beads through isourea linkage.
CellMosaic's SepSphereÔ protein A agarose has been used routinely in house for purification of antibodies and antibody conjugates, demonstrating consistent performance and quality.
Figure 1: Binding Specificity of Protein A Agarose.
10 µl Protein A Agarose beads were incubated at RT for 2 hours with 40 µl PBS buffer containing 1 µg/µl of human IgG and 0.5 µg/µl of thyroglobumin, bovine serum albumin, ovalbumin, carbonic anhydrase, and RNase A each. The input and supernants (5 ul) were run on a 4-12% Bis-Tris SDS-PAGE (Invitrogen) with 1X MES running buffer and stained with Simply Blue (Sigma).