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SEC (Gel filtration) HPLC Protein Standard (8 components)

  • SEC HPLC profile of CellMosaic's protein standard
Price:
$149.00
SKU:
CM92001
Availability:
In stock, usually ships within 24 hrs
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Product Description

 

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Product Sheet COA-s91203

Product Description

CellMosaic’s size exclusion chromatography (SEC) or gel filtration protein standard is designed for day to day usage to check HPLC column performance and the analysis of bioconjugates. This product consists of 7 proteins and one blue dextran with a MW range of 6 to 2000 KDa. The broad range of the protein molecular weight coverage allows easy identification of small molecules, conjugates, and aggregates in a bioconjugation mixture, such as antibody-drug conjugation, protein, and antibody modification and conjugation. The product is validated in many of the commonly used SEC columns. CellMosaic routinely uses this product for its internal bioconjugation-related research.

A total of four micro-centrifuge tubes are in a package. Each micro-centrifuge tube contains 80 µL of frozen protein standards solution. The product is ready for use after thawing. 


Application of the product:

  • Calibration of gel filtration and size exclusion column
  • Determination of the size and molecular mass of proteins
  • Analysis of small molecules, bioconjugates, and aggregates

Key Features of the product:

  • Frozen solution and ready to use for HPLC analysis after thawing
  • Mixture of 8 high purity proteins with broad MW coverage: 6 KDa to 2000 KDa

 Composition

# Name MW (KDa)
1     Aprotinin (bovine lung) 6.5
2     RNase A 13.7
3     Carbonic anhydrase 29
4     Ovalbumin (albumin chicken egg) 43
5     Aldolase (rabbit muscle) 158
6     Ferritin (equine spleen) 440
7     Thyroglobulin 669
8     Blue Dextran 2000

 

 

 

 

 

 

 

 


Application Note 

Calibration of Protein and Conjugate MW

The approximate MW of an unknown protein or protein conjugate can be determined by comparing its retention time to the protein standard (Figures 1-4 give examples of the SEC profile of some commonly used SEC columns). For more accurate calculation, an SEC MW standard curve is recommended (Figure 3). 

Figure 1: SEC HPLC profile of protein standards in a Shodex Protein KW803 column (8.0 mm ID x 300 mm, S/N E1390022). HPLC condition: isocratic PBS buffer at 0.5 mL/min. Instrument: Agilent 1100 HPLC series.  

 KW803 column SEC profile

Figure 2: SEC HPLC profile of protein standards in a TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, S/N M0077-504G). HPLC condition: isocratic PBS buffer at 0.5 mL/min. Instrument: Agilent 1100 HPLC series.

TSKgel BioAssist G3SWxl SEC profile

 

Figure 3: SEC MW standard curve of TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, S/N M0077-504G). Data from Figure 2.

SEC MW standard curve

Figure 4: SEC HPLC profile of protein standards in a Waters BioSuite 250 4 µm UHR SEC (4.6 mm x 300 mm, S/N: 081A15286VE029). HPLC condition: isocratic PBS buffer at 0.35 mL/min. Instrument: Agilent 1100 HPLC series.

Waters BioSuite UHR SEC profile

 

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