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Protein Mertansine (DM1) Conjugation Kit

  • Protein DM1 conjugate CM11414
  • Mertansine (DM1) Chemical Structure
Price:
$990.00
SKU:
CM11414
Availability:
Custom kit made to order, usually ships within 1 week
Shipping:
Calculated at checkout
Quantity:


 

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SDS USER MANUAL CM11414

CellMosaic® has designed this personalized conjugation kit to work with any protein with a MW over 20 KDa. The customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a maelimide-protein via SMCC (succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate) linker, followed by reaction of the maleimide-protein with Mertansine (DM1) to generate the conjugates (a method developed by Immunogen). One step purification typically provides the resulting protein-DM1 at greater than 95% purity. 

This kit provides materials to label either one or three proteins. Reaction scale: 20 nmol 

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Requirement for protein:

  • Amount: 20 nmol (1 mg if the MW of protein is 50KDa) (Lower amount of protein is fine, but need customization. Please contact us for the customization)
  • MW: >20 KDa (Lower MW is fine, but may need customization or custom synthesis. Please contact us for any suggestion)
  • Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
  • Protein has some lysine residues

Drug Information

  • Name: Mertansine (DM1) 
  • CAS number: 139504-50-0
  • Mechanism of action: Inhibits cell division by blocking the polymerisation of tubulin

Key Features of this PerKit™

  • Offers a simple and easy way to label protein with DM1 with minimum exposure to the toxin
  • Stable linkage 
  • Fast and easy preparation: 6 h preparation and <2 h hands-on time
  • All reagents and supplies included for preparation and purification
  • Average 2-4 DM1 labeling per protein 
  • Options to choose tailored services at CellMosaic after conjugation

Prior to conjugation: you can supply your protein information when you place your order. CellMosaic will give recommendations for the conjugation if your protein has special features.

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DOL.

 


Related Products

Maleimide Assay Kit (Cat. CM90002): The average maleimide groups per antibody is 4 after SMCC labeling. If necessary, you can purchase this kit separately from CellMosaic to assay the maleimide content. The kit is simple and easy to use. Approximate total assay time is 1 h.

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