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PerKit™ Small Molecule Acid by Cy5

  • Small Molecule Acid labeling by Cyanine 5 dye
Price:
$500.00
SKU:
CM62403
Availability:
On stock, usually ships within 24 hrs
Shipping:
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Product Description

 

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SDS USER MANUAL

This is a Personalized conjugation kit (PerKit™) for Cyanine 5 dye labeling of a small molecule containing carboxylic acid (-COOH) functional groups (1 mg scale). Using the kit components, the user converts the carboxylic acid (not supplied) to an activated N-hydroxysuccinimide ester (NHS ester), followed by reaction with an equivalent amount of the amine cyanine 5 dye. 


Requirement for Small Molecule

  • Preferably > 90% pure
  • Absence of primary or secondaryamine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

  • Offers a simple and easy way to label small molecules with COOH: 2hr preparation and <30 minutes hands-on time
  • Quantitative reaction with stable bond
  • Options to choose tailored services at CellMosaic prior and after conjugation

Prior conjugation, you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendation for the conjugation if there is some special feature of your molecule. 

After conjugation, you can choose to send your sample to CellMosaic for HPLC and MS analysis.


Protocol

 cm62403-protocol.jpg

 


Labeling Chemistry

cm62403-labeling-chemistry2.png

 


Spectral properties

Excitation maximum: 646 nm
Extinction coefficient (ε): 250,000 L⋅mol−1⋅cm−1
Emission maximum: 662 nm
Fluorescence quantum yield: 0.2 

Typical Kit Performance Data (LC Analysis, CellMosaic)

Figure 1: C18 HPLC/UV analysis of cyanine 5 amine (detected at 640 nm)

cm62403-hplc-cyanine-png.png

Figure 2: C18 HPLC/UV analysis of the reaction mixture of cyanine 5 labeling of Fmoc-Gly-OH (1:1 ratio). All cyanine reacted. A single product peak detected at 640 nm demonstrates the characteristic UV spectra of the Fmoc group and cyanine (detected at 640 nm)

cm62403-hplc-cyanine-labeling-of-sm-png.png

 

 

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