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PerKit™ Small Molecule Acid by Cy5

  • Small Molecule Acid labeling by Cyanine 5 dye
Price:
$500.00
SKU:
CM62403
Availability:
On stock, usually ships within 24 hrs
Shipping:
Calculated at checkout
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SDS USER MANUAL

This is a Personalized conjugation kit (PerKit™) for Cyanine 5 dye labeling of a small molecule containing carboxylic acid (-COOH) functional groups (1 mg scale). Using the kit components, the customer converts the carboxylic acid (not supplied) to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with an equivalent amount of the amine cyanine 5 dye. 


Requirement for Small Molecule

  • Preferably > 90% pure
  • Absence of primary or secondaryamine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

  • Offers a simple and easy way to label small molecules with COOH: 2 h preparation and <30 minutes hands-on time
  • Quantitative reaction with stable bond
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendations for the conjugation if your molecule has special features. 

After conjugation: you can choose to send your sample to CellMosaic for HPLC and MS analysis.


Protocol

 cm62403-protocol.jpg

 


Labeling Chemistry

cm62403-labeling-chemistry2.png

 


Spectral properties

Excitation maximum: 646 nm
Extinction coefficient (ε): 250,000 L⋅mol−1⋅cm−1
Emission maximum: 662 nm
Fluorescence quantum yield: 0.2 

Typical Kit Performance Data (LC Analysis, CellMosaic)

Figure 1: C18 HPLC/UV analysis of cyanine 5 amine (detected at 640 nm)

cm62403-hplc-cyanine-png.png

Figure 2: C18 HPLC/UV analysis of the reaction mixture of cyanine 5 labeling of Fmoc-Gly-OH (1:1 ratio). All cyanine reacted. A single product peak detected at 640 nm demonstrates the characteristic UV spectra of the Fmoc group and cyanine (detected at 640 nm)

cm62403-hplc-cyanine-labeling-of-sm-png.png

 

 

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