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PerKit™ Small Molecule Acid by Cy3

  • Small molecule acid labeling by Cyanine 3 dye
Price:
$500.00
SKU:
CM62401
Availability:
Made to order, usually ships within 3-5 days
Shipping:
Calculated at checkout
Describe your small molecule:

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Or upload your small molecule structure:

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SDS USER MANUAL

This is a Personalized conjugation kit (PerKit™) for Cyanine 3 dye labeling of a small molecule containing carboxylic acid (-COOH) functional groups (1 mg scale). Using the kit components, the customer converts the carboxylic acid (not supplied) to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with an equivalent amount of the amine cyanine 3 dye. 


Requirement for Small Molecule

  • Preferably > 90% pure
  • Absence of primary or secondaryamine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

  • Offers a simple and easy way to label small molecules with COOH: 2 h preparation and <30 minutes hands-on time
  • Quantitative reaction with stable bond
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendation for the conjugation if your molecule has special features. 

After conjugation: you can choose to send your sample to CellMosaic for HPLC and MS analysis.


Protocol

 cm62401-protocol.png

 


Labeling Chemistry

cm62401-scheme.png

 


Spectral Properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31 

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