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PerKit™ Protein Small Molecule Acid Conjugation Kit

Price:
$314.00
SKU:
CM52408
Availability:
Made to order, usually ships within 3-5 days
Shipping:
Calculated at checkout
Describe your small molecule:

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Product Description

 

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SDS USER MANUAL

This is a Personalized conjugation kit (PerKit™) for protein labeling of a small molecule containing carboxylic acid (-COOH) functional groups (50 nmole scale, 2 mg total for a 40KDa protein). Using the kit components, the user converts the carboxylic acid to an activated sulfo N-hydroxysuccinimide ester (NHS ester), followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is desalted to remove any unreacted small molecule acid. 


Requirement for Small Molecule

  • Preferably > 90% pure
  • Absence of primary or secondary amine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

  • Offers a simple and easy way to label protein with any small molecule containing carboxylic acid
  • Fast and easy preparation: 4hr preparation and <30 minutes hands-on time
  • All reagents and supplies included for preparation and purification
  • Over 90% pure conjugated products by SEC
  • Options to choose tailored services at CellMosaic prior and after conjugation

Prior conjugation, you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendation for the conjugation if there is some special feature of your molecule. 

After conjugation, you can choose to send your sample to CellMosaic for HPLC analysis.


Protocol

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Labeling Chemistry

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