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PerKit™ HRP-Peptide Conjugation Kit

  • HRP peptide conjugates
Price:
$197.00
SKU:
CM32401x1
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Peptide Information: MW, sequence:

(Maximum Size: . Type: )
Quantity:


 

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SDS USER MANUAL

CellMosaic® has designed this personalized conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepares the HRP-peptide conjugate by reacting their peptide (customer supplied) with activated HRP. One step purification typically provides the resulting HRP-peptide at greater than 95% purity.

This kit provides materials to label one to three peptides. Total amount of activated HRP included in a reaction: 2 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Requirement for Cys peptide:

  • Amout: 1-5 mg depending on the peptide MW
  • HPLC purified and lyophilized. Please make sure there is no reducing reagents, such as DTT, are present. The Cys peptide should be stored at -80 degress Celsius.
  • HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this HRP-Peptide Conjugation Kit:

  • High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
  • Optimal maleimide groups per HRP for single-label peptide: 1.2 for typical batch
  • A single purification step affords over 95% of the majority of single-labeled HRP-peptide conjugates 
  • Fast preparation: less than 1 h hands-on time
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order, and CellMosaic will give recommendations for the conjugation if your peptide has special features

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates


 Labeling Chemistry

 cm32401-hrp-peptide-scheme.png

 

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