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PerKit™ BSA Small Molecule Acid Conjugation Kit

  • BSA Small Acid Conjugates
Price:
$390.00
SKU:
CM52403
Availability:
In stock, usually ship within 1-3 days
Shipping:
Calculated at checkout
Describe your small molecule:

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SDS USER MANUAL

Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. BSA is often used as a carrier protein for immunization. CellMosaic® has designed this personalized BSA conjugation kit to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

This kit provides materials to perform one BSA labeling. Total amount of BSA included: 5 mg.


Requirement for Small Molecule

  • Preferably > 90% pure
  • Absence of primary or secondary amine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

  • Offers a simple and easy way to label BSA with any small molecule containing carboxylic acid
  • Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
  • All reagents and supplies included for preparation and purification
  • Over 90% pure conjugated products by SEC
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendations for the conjugation if your molecule has special feature. 

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.


Protocol

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Labeling Chemistry

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