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Perkit™ Antibody-Oligo Conjugation Kit

  • Antibody oligo conjugation kit
Price:
$499.00
SKU:
CM53405
Availability:
Customer kit made to order, usually ships within 1-2 weeks
Shipping:
Calculated at checkout
Quantity:


 

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SDS USER MANUAL

CellMosaic® has designed this personalized antibody-oligo conjugation kit to work with any antibody and an amine-modified oligos. The user supplies its own amine modified oligo, which are readily available from many commercial oligo suppliers. The customer also supplies the antibody. Using the kit components, the customer converts the amine-oligo to a maleimide-oligo, followed by reaction of a thiol modified antibody to generate the conjugates. One step purification step typically provides the resulting antibody-oligo at greater than 80% purity. 

This kit provides materials to perform one to three conjugations. Scale of the reaction based on antibody: 1 mg.

The kit materials will be supplied based on your oligo length. Please supply this information when you places the order. Note: If the oligo is over 30 mer, the kit may not be very efficient. CellMosaic will supply a quote for a custom synthesis.


Requirement for antibody:

  • Amount: 1 mg 
  • Preferably >90% pure by gel electrophoresis 

Requirement for amine-oligo:

  • Amount: 50 nmol 
  • Oligo length: <30 mer
  • HPLC purified and lyophilized
  • Additional components of acid or other molecules containing primary or secondaryamine groups and thiol should NOT be present 

Key Features of this Peptide-Oligo Conjugation Kit:

  • Offers a simple and easy way to prepare antibody-oligo conjugate
  • Single label 
  • Fast and easy preparation: 6 h preparation and <3 h hands-on time
  • All reagents and supplies included for preparation to purification
  • Options to choose tailored services at CellMosaic prior to and after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.

 

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