This strategy utilizes an N-terminal Cys incorporated peptide to react with a maleimide activated oligo. If the peptide has an internal Cys, we can use other strategies such as oxime formation through a hydroxyl-amine modified peptide reaction with an aldehyde modified oligo. Although, the price for using other strategies for making peptide-oligo conjugate is the same, the price for obtaining other modified peptides may be higher.

• Chemistry:  Amine-oligo is first activated with an NHS ester-maleimide heterobifunctional crosslinker, then reacts with a terminal Cys containing peptide.
• Specification: Product is HPLC purified and usually over 90% pure.
• Procedure:  After a buffer exchange (if necessary), labeling of amine modified-oligo with NHS ester-maleimide followed by gel filtration to remove excess crosslinking reagents. Then conjugation of peptide and HPLC purification. Finally concentration determination, HPLC and MS analysis.
• Price: Standard price covers labor and materials including the cost of maleimide -activated oligo preparation, Cys-peptide conjugation procedure, conjugate purification, and analysis.
• Starting materials: Please check biopolymer for ordering information.