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Oligo—HRP conjugate

  • Image 1
  • Scheme: thiol modified enzyme reaction with a maleimide activated oligo
  • Example 1: Synthesis and purification of single labeled oligo-HRP conjugates
Price:
Please contact us for a quote.
SKU:
BC0008


Product Description

CellMosaic is the preferred choose for diagnostic companies to source highly pure and single labeled oligo-HRP conjugates.

  • Chemistry: HRP and oligo linked together through a thiol ether bond. In many cases, a long PEG crosslinker may be used for the flexibility and preservation of HRP activities.
  • Specification: Single label or a pool of heterogeneous DOL products depending on customer's request.
  • Procedure: After a buffer exchange (if necessary), labeling of amine modified-oligo with NHS ester-maleimide followed by gel filtration to remove excess crosslinking reagents.Then thiolation of HRP followed by conjugation of HRP and gel filtration. Finally concentration determination and gel electrophoresis analysis of oligo-HRP conjugates. Different purification strategies may be used for large scale oligo-HRP conjugates.
  • Price: Contact us for a quote.
  • Starting materials: Please check biopolymer for ordering information.
  • Discount: 15 % discount price applies to additional conjugates ordered at the same time

Examples (see images):

  1. Synthesis and purification of a single labeled oligo-HRP conjugates at CellMosaic (shown below): >98% pure by size exclusion HPLC, characteristic UV spectrum of HRP (410 nm) and oligo (260 nm).

     

    bc0008-oligo-hrp-example.jpg
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