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HRP Oligo Conjugation Kit (Single Label)

  • CM53401x3 HRP Oligo Conjugation Kit
Price:
$197.00
SKU:
CM53401x1
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Product Description

 

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SDS USER MANUAL

CellMosaic has designed this HRP-Oligo conjugate with linkers and conjugation chemistry that retain the activity of HRP. The same configuration and chemistry have been tested for HRP-Oligos with different oligo sequences in several diagnostic companies since 2011.

This HRP-Oligo Conjugation kit works with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity. 

This kit provides materials to perform HRP labeling of one (CM53401x1) or three oligo samples (CM53401x3). Scale of the reaction: 5 to 10 nmole oligo.


Key Features of this HRP-Oligo Conjugation Kit:

  • High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
  • Optimal maleimide groups per HRP for single label of oligo:  1.2 for typical batch
  • A single purification step affords 85~90% of single labeled HRP-oligo conjugates 
  • Fast preparation: less than 2 h hands-on time
  • Easy purification: less than 30 minutes
  • Stable linkage with optimal PEG spacer to make sure there is no interference between oligo binding and HRP activation
  • No activity loss of the  HRP after the conjugation and purification
  • All reagents included, from preparation to purification

 Labeling Chemistry

scheme.jpg

 

 


Typical Kit Performance Data (LC Analysis, CellMosaic)

Oligo information: HPLC-purified 18mer oligo with 5’ NH2-C6 modification

Figure 1: Size exclusion HPLC analysis of the HRP and oligo reaction mixture (before purification).

figure1-cm53401.jpg

 

Figure 2: Size exclusion HPLC analysis of first elution (E1) from Column Q (after purification).

figure2-cm53401.jpg

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