IgG molecule contains a number of disulfide bonds. The disulfide bonds in the hinge region can be selectively reduced to generate a half antibody containing sulfhydryl groups. This procedure generates limited reactive sites for antibody labeling through thiol.

• Chemistry: Sulfhydryl reaction with maleimide or alkyl halide containing compounds.
• Product: Labeled half antibody containing single or few small molecules.
• Procedure: After a buffer exchange (if necessary) disulfide reduction of IgG followed by desalting and labeling. After labeling, small molecule labeled half antibodies are purified by gel filtration chromatography. Finally concentration/labeling ratio determination.
• Price: Standard price includes materials and labor for making half anitibody and labeled half antibody.
• Note: For antibodies other than IgG extra charges may apply. Please check biopolymer and small molecule for ordering information.

References:

1. Cole, R. D.; Stein, W. H.; Moore, S. On the cysteine content of human hemoglobin. J. Biol. Chem. 1958 Dec; 233(6):1359-63. PMID: 13610843
2. Gurd, F. R. N. Carboxymethylation. Methods in Enzymology. 1967, 11, p532. Academic Press, New York.
3. Smyth, D. G.; Blumenfeld, O. O.; Konigsberg, W. Reactions of N-ethylmaleimide with peptides and amino acids. Biochem. J. 1964 Jun; 91(3):589-95. PMID: 5840721
4. Gorin, G.; Martic, P. A.; Doughty, G. Kinetics of the reaction of N-ethylmaleimide with cysteine and some congeners. Arch. Biochem. Biophys. 1966 Sep 9; 115(3):593-7. PMID: 5970483