In this case sulfhydryl functional groups will be introduced through the reduction of F(ab')2 fragments. Conjugates produced by this method retain high activity.

• Chemistry: F(ab')2 fragments are reduced at their heavy chain disulfides and then conjugated with a maleimide-activated enzyme HRP.
• Specification: A pool of low molecular weight heterogeneous products.
• Procedure: After a buffer exchange (if necessary), labeling of HRP (the enzyme) with NHS ester-maleimide followed by gel filtration to remove excess crosslinking reagents. Then reduction of F(ab')2 followed by conjugation of Fab' to the labeled HRP and purification. Finally, concentration determination and gel electrophoresis analysis of Fab'-HRP conjugate.
• Price: Standard price covers labor and materials including the cost of HRP and its labeling, reduction of F(ab')2 fragments, conjugation procedure, conjugate purification and analysis. For antibodies other than IgG, extra charges may apply.
• Starting materials: Please check biopolymer for ordering information.
• Discount: 15 % discount price applies to addtional conjugates ordered at the same time.