The standard price is $2000 for 3 mg of starting biopolymers. Please contact us for a quotation.
Chemistry: Typical preparation of antibody conjugates using NHS ester-Maleimide heterobifunctional crosslinkers.
Specification: A pool of heterogeneous products.
Procedure: After a buffer exchange (if necessary), labeling of HRP (the enzyme) with the crosslinking reagent followed by gel filtration to remove excess crosslinking reagents. Then antibody thiolation using 2-iminothiolane followed by conjugation of antibody to the labeled HRP and purification. Finally, concentration determination and gel electrophoresis analysis of the Antibody-HRP conjugate.
Price: Standard price covers labor and materials including cost of HRP and its labeling, antibody modification, conjugation procedure, conjugate purification, and analysis. For antibodies other than IgG, extra charges may apply.
Starting materials: Please check biopolymer for ordering information.
Note: In this case sulfhydryl functional groups are introduced to an antibody through Lys residues using a reliable and reproducible procedure. However, if some of the modified or conjugated Lys residues are located on the antigen binding sites, this method may produce partially active or inactive antibody-enzyme conjugates that may not bind to the antigen. In such a case an alternative conjugation method may be used.
Discount: 15 % discount price applies to addtional conjugates ordered at the same time.