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Antibody-Drug Conjugate (ADC) Kits and Solution

CellMosaic ADC PerKits™ are available to prepare antibody conjugates through surface amines or reduced thiols. Some ADC kits apply to general payloads with certain functional groups. Some ADC kits supply the selected, standard linkage molecules conjugated to a selected payload, ready with the appropriate reactive modifications to directly attach the payload and linker to your specific antibody of interest. The kits are complete with all reagents and procedures for attaching the selected payload directly to your antibody and purifying the final ADC. All you need to supply is your antibody and standard lab equipment.

A typical CellMosaic personalized ADC kit results in the preparation of approximately 2-3 mg of final ADC in buffer with a target drug-to-antibody ratio (DAR) of 2 to 6, averaging a DAR of 3-4. Purities are typically over 90% for the conjugates with free or less than 5% of the free payload. Individual results can vary depending on the specific antibody system you are using and the hydrophobicity of your payload. Kit instructions are complete and easy to follow, and can typically be carried out within 4 hours (with less than 1-hour hands-on time).

Other products on the market for antibody screening may utilize a general secondary antibody, or a general antibody affinity coupling (such as protein-A), to provide indirect conjugation, but these indirect conjugation approaches for ADCs can significantly impact the hydrodynamic size and physiological behaviors (for example, changing cleavage and degradation mechanisms), so these products do not provide a fully representative approach for evaluating your novel ADC in screening or for proof-of-concept (POC) studies.

 

CellMosaic currently provides the following ADC Perkits™, and we are adding additional kits configurations as needed. Please inquire if you do not see your current configuration of interest listed.