Native proteins, oligos, and peptides have no aldehyde functional group. Introduction of aldehyde groups into these biopolymers via diol oxidation allows them to react with other molecules specifically at the aldehyde site through reductive amination, oxime, and hydrazone formation.

• Chemistry:  Aldehyde introduction through periodate oxidation of carbohydrates or molecules containing diols.
• Specification: Only one or few aldehyde groups are introduced onto the biopolymer depending on the nature of biopolymer and the extent of the oxidation.
• Procedure:  After a buffer exchange (if necessary), periodate oxidation followed by gel filtration. Finally concentration determination and testing.
• Price: Standard price covers labor and materials for the modification procedure.
• Starting materials: Please check if your biopolymer contains any sugar or diol residues. For ordering information, please visit biopolymer for ordering information.

References:

1. Bobbit, J. M. Periodate oxidation of carbohydrates. Adv. Carbohydr. Chem. 1956; 48(11):1-41. PMID: 13469627
2. Rothfus, J. A.; Smith, E. L. Glycopeptides. IV. The periodate oxidation of glycopeptides from human gamma-globulin. J. Biol. Chem. 1963; 238(4):1402-10. PMID: 13975369
3. Lotan, R.; Debray, H.; Cacan, M.; Cacan, R.; Sharons, N. Labeling of soybean agglutinin by oxidation with sodium periodate followed by reduction with sodium [3-H]borohydride. J. Biol. Chem. 1975; 250(5):1955-7. PMID: 163260
4. Van Lenten, L.; Ashwell, G. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins. J. Biol. Chem. 1971; 246(6):1889-94. PMID: 4323238
5. Wilchek, M.; Bayer, E. A. Labeling glycoconjugates with hydrazide reagents. Methods Enzymol. 1987; 138:429-42. PMID: 3110546